Guo, Xing Rong Wang, Xiao Li Li, Man Chol Yuan, Ya Hong Chen, Yun Zou, Dan Dan Bian, Liu Jiao Li, Dong Sheng. In vivo messenger RNA introduction into the central nervous system using polyplex nanomicelle. Uchida, Satoshi Itaka, Keiji Uchida, Hirokuni Hayakawa, Kentaro Ogata, Toru Ishii, Takehiko Fukushima, Shigeto Osada, Kensuke Kataoka, Kazunori. Feeder-free derivation of human induced pluripotent stem cells with messenger RNA. Warren, Luigi Ni, Yuhui Wang, Jiwu Guo, Xirong. Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands. Moody, Krishna-Sulayman Debatis, Michelle Marshak-Rothstein, Ann. Pseudouridine in RNA: what, where, how, and why. Increased erythropoiesis in mice injected with submicrogram quantities of pseudouridine-containing mRNA encoding erythropoietin.Ĭharette, M. Karikó, Katalin Muramatsu, Hiromi Keller, Jason M. Nucleofection induces transient eIF2α phosphorylation by GCN2 and PERK. Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA.Īnderson, B. Karikó, Katalin Muramatsu, Hiromi Ludwig, János Weissman, Drew. Nucleoside modifications in RNA limit activation of 2'-5'-oligoadenylate synthetase and increase resistance to cleavage by RNase L. Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation.Īnderson, Bart R. Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA.Īnderson, Bart R. Karikó, Katalin Buckstein, Michael Ni, Houping Weissman, Drew. Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. Ludwig, János Kato, Hiroki Akira, Shizuo Weissman, Drew. Karikó, Katalin Muramatsu, Hiromi Welsh, Frank A. Predominant and distinct roles of the mnm5 and s2 modifications of U34. Effect of modified nucleotides on Escherichia coli tRNAGlu structure and on its aminoacylation by glutamyl-tRNA synthetase. everybody I know who is making pseudoU RNA is getting it from you." The product is very good and the customer service is very good too. Katalin Karikó, prominent mRNA researcher, references TriLink as a leading manufacturer of Pseudo-UTP "We value TriLink, it is an excellent company indeed. Reduced toxicity due to substitution with Pseudo-UTP, 5-Methyl-CTP was critical since it allowed repeated transfection with in vitro transcribed mRNA over several weeks. found that enzymatically synthesized RNA substituted with Pseudo-UTP, 5-Methyl-CTP and ARCA effectively evaded the cell’s innate immune response, a crucial component in their success. These cells are referred to as induced pluripotency stem cells (iPSCs). determined an efficient means of reprogramming multiple human cell types using modified mRNA that can express the four primary reprogramming proteins. Pseudo-UTP, along with 5-methylcytidine-5'-triphosphate (5-methyl-CTP) has shown innate immune suppression in culture and in vivo while enhancing translation in recent publications. Pseudouridine-5'-triphosphate (Pseudo-UTP) is used to impart desirable mRNA characteristics such as increased nuclease stability, increased translation or altered interaction of innate immune receptors with in vitro transcribed RNA. Pseudouridine has been found to enhance base stacking and translation. It can be found in structural RNAs, such as transfer, ribosomal and small nuclear RNA. It is the most abundant natural modified RNA base, and has been deemed the "fifth nucleoside" in RNA. Pseudouridine (5-ribosyluracil) was the first modified ribonucleoside discovered.
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